Abcam, ab209665, Anti-Brachyury / Bry antibody [EPR18113]

Size: 20µL / 100µL / 1mL
Anti-Brachyury / Bry antibody [EPR18113] (ab209665) is a rabbit monoclonal antibody detecting Brachyury / Bry in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC- Wmt, ICC/IF . Suitable for Human, Mouse, Rat . - Clone EPR18113 is the most cited clone to Brachyury / Bry - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18113,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, IP, Flow Cyt (Intra), WB, IHC - Wmt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Brachyury / Bry antibody [EPR18113] (ab209665) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC - Wmt, IHC-P, IP, WB in human, mouse, rat samples.
Anti-Brachyury / Bry antibody [EPR18113] (ab209665) has been cited over 38 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Brachyury / Bry antibody [EPR18113] (ab209665) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Brachyury / Bry antibody [EPR18113] (ab209665) has 9 independent reviews from customers.
Anti-Brachyury / Bry antibody [EPR18113] (ab209665) specifically detects Brachyury / Bry (UniProt ID: O15178; Molecular weight: 47kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR18113 -
ab236023
Antibody clone EPR18113 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 555 (
ab314674
ab314675
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Brachyury protein also known as Bry or T acts as a transcription factor and belongs to the T-box family of genes. It consists of about 435 amino acids giving it an estimated mass of around 47 kDa. Brachyury plays a critical role in the development of the posterior mesoderm and nucleus pulposus during embryogenesis. It is strongly expressed in the notochord mesoderm and some tumor tissues. The protein's presence can be detected using specific assays such as Brachyury IHC (immunohistochemistry) and Brachyury stain which help identify Brachyury marker expression in both normal and pathological states.
Biological function summary
The Brachyury protein influences the morphogenetic movement in mesoderm development. It directly interacts with specific DNA sequences to regulate the transcription of target genes. Brachyury often operates as part of a larger transcriptional complex that manages the differentiation and migration of cells during embryogenesis. The gene's expression indicates mesodermal differentiation and is important for cell fate decisions within developing embryos.
Pathways
Brachyury plays a significant role in the Wnt signaling and the TGF-beta pathways. These pathways are essential in controlling cell proliferation and differentiation. Brachyury directly impacts the regulation of these pathways influencing other proteins like beta-catenin in Wnt signaling. This interconnectedness suggests its broader role in cellular communication and development processes potentially affecting gene expression across these pathways.
Brachyury has been associated with various cancers such as chordoma and other related tumors. Chordoma specifically involves the notochord where Brachyury marks the disease's presence. Other proteins like E-cadherin interact with Brachyury in tumor progression and metastasis. Understanding Brachyury's function helps in diagnosing and potentially targeting treatment options for these ailments.