Abcam, ab209881, Human sTNF RI ELISA Kit

Size: 1 x 96Tests
Human sTNF RI ELISA Kit is a single-wash 90-min Simplestep used to quantify Human sTNF RI with a sensitivity of 1.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.9 pg/mL,
Range:15.6 - 1000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Human sTNF RI ELISA Kit ab209881 is a rapid single-wash 90-min Sandwich ELISA to measure Human sTNF RI in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 1.9 pg/mL.
How the assay works
Human sTNF RI SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human sTNF RI ELISA Kit ab209881 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Soluble TNF Receptor I antibody [EPR18822-47] - BSA and Azide free (Capture)
ab242762
- Anti-Soluble TNF Receptor I antibody [EPR18822-13] - BSA and Azide free (Detector)
ab242762
Tumor necrosis factor receptor superfamily member 1A (TNF RI) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RI. The standard protein in this kit is soluble TNF RI and the capture and detector antibodies were raised against this region of TNF RI.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Soluble TNF Receptor I + Fc Fragment also known as TNFRSF1A-Fc is a recombinant fusion protein with an approximate mass of 75 kDa. Mechanically this fusion protein acts by binding to tumor necrosis factor-alpha (TNF-α) inhibiting its activity and preventing interactions with cell surface TNF receptors. Soluble TNF Receptor I + Fc Fragment keeps the TNF-α activity in check by acting as a decoy receptor. It is expressed in various cells including immune cells and endothelial cells allowing it to modulate inflammatory responses effectively.
Biological function summary
The Soluble TNF Receptor I + Fc Fragment helps regulate immune and inflammatory responses. It functions by blocking the pro-inflammatory effects of TNF-α an element pivotal in the inflammatory process. By neutralizing TNF-α it prevents the recruitment and activation of immune cells and cytokines. This protein does not function alone; it works within larger protein complexes to execute its role in cellular communication and signal transduction.
Pathways
This fusion protein plays a significant role in the NF-κB and apoptosis signal pathways. Within these pathways TNF-α is an important mediator whose activity is moderated by the presence of the Soluble TNF Receptor I + Fc Fragment. By inhibiting TNF-induced activation of NF-κB the fusion protein can influence processes related to cell survival and death. It is closely related to proteins such as IκB (inhibitor of NF-κB) showing how its activity can have downstream effects on a variety of signaling processes.
Soluble TNF Receptor I + Fc Fragment is used therapeutically in conditions like rheumatoid arthritis and inflammatory bowel disease. These disorders involve high levels of TNF-α which contribute to excessive inflammatory responses. The fusion protein helps mitigate such conditions by decreasing the TNF-α activity which in turn lowers inflammation. Through these actions this target is also indirectly connected to the regulatory proteins involved in these disorders like interleukin-6 (IL-6) which can also influence the inflammatory process.