Abcam, ab210029, Alexa Fluor® 405 Anti-PDHB antibody [EPR11097(B)]

Size: 100µL
Rabbit Recombinant Monoclonal PDHB antibody - conjugated to Alexa Fluor® 405. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11097(B),
Isotype:IgG,
Conjugation:Alexa Fluor® 405,
Excitation/Emission:Ex: 401nm, Em: 421nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDHB also known as pyruvate dehydrogenase beta subunit plays an important role in the conversion of pyruvate to acetyl-CoA. This protein forms a part of the pyruvate dehydrogenase complex (PDC) which occurs in mitochondria of numerous tissues. The PDHB subunit has a molecular mass of approximately 39 kDa. Expression of PDHB is not limited to one specific type of tissue it is generally found in various tissues indicating its widespread involvement in cellular metabolism.
Biological function summary
PDHB functions as an essential component of the PDC which catalyzes the decarboxylation of pyruvate linking glycolysis to the citric acid cycle. The PDC itself is a large multi-enzyme complex including other subunits such as E1 and E2 which coordinate efficiently to carry out its function. In this way PDHB contributes to energy production by enabling entry of carbon skeletons into the tricarboxylic acid cycle important for ATP generation.
Pathways
PDHB holds a significant role in central metabolic pathways namely glycolysis and the citric acid cycle. The conversion facilitated by the PDC forms a vital cornerstone for metabolic flux regulation influencing gluconeogenesis and fatty acid synthesis. PDHB also interacts with proteins such as pyruvate dehydrogenase kinase which regulates the PDC activity by phosphorylation thereby affecting energy metabolism directly.
Alterations in PDHB can lead to metabolic disorders such as pyruvate dehydrogenase deficiency potentially causing neurological dysfunction due to impaired energy metabolism. PDHB mutations also have a link to mitochondrial diseases reflecting its significant role in energy homeostasis. In these contexts proteins like the pyruvate dehydrogenase phosphatase are involved highlighting the clinical relevance of PDHB alterations in maintaining proper metabolic health.