Abcam, ab210204, Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free

Size: 100µg
Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204) is a rat monoclonal antibody provided in an azide free buffer detecting Ly6g in Flow Cytometry, IP, IHC-P, IHC-Fr, FuncS . Suitable for Mouse . - Suitable for in vitro and in vivo studies - Over 10 publications
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:1A8,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Mouse,
Applications:IHC-Fr, IP, Flow Cyt, IHC-P, FuncS (Depl)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What is this antibody validated in?
Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204) is a rat monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Functional Studies in Mouse samples.
Trusted by the scientific community
Anti-Ly6g [1A8] - Low endotoxin, Azide free (ab210204) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.
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Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-ab210204 was purified from tissue culture supernatant via affinity chromatography., Storage buffer-pH: 7.2Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.
Biological function summary
Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.
Pathways
Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.
Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.