Abcam, ab210554, Anti-HuC/HuD protein antibody

Size: 100µL
Rabbit Polyclonal HuC/HuD protein antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ELAVL3 aa 1-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Zebrafish,
Applications:IHC - WmtSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ELAVL3 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.Q14576

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HuC/HuD proteins often referred to as HuC/D markers or HuC protein are part of the ELAV (embryonic lethal abnormal visual system) family. These proteins are RNA-binding and weigh approximately 40kDa. They show expression mainly in neuronal tissues acting as post-transcriptional regulators within neurons. HuC/D proteins are used as markers for neural differentiation particularly in studies involving developing neural tissues or cultured neurons such as those in HuC zebrafish models.
Biological function summary
These RNA-binding proteins regulate mRNA stability and translation thereby influencing neuronal differentiation and function. HuC/D proteins do not operate as isolated units; instead they form complexes with various RNA molecules. These complexes facilitate the stabilization and transport of mRNAn important for the synthesis of certain neuronal proteins. Combined activity of HuC and HuD is significant in maintaining cellular identity in neurons.
Pathways
HuC/D proteins play roles in neuronal differentiation and maintenance pathways. One of these pathways is the MAPK/ERK pathway which is involved in transmitting chemical signals from the cell surface to the DNA in the cell nucleus and affects neuron survival and growth. The function of HuC/D within this pathway connects them to other proteins like NeuroD and N-myc which are related in the regulation of neural gene expression.
HuC/D proteins have associations with neurodegenerative conditions such as Alzheimer’s disease and Parkinson’s disease. The improper regulation of RNA-binding proteins like HuC/D can lead to neuronal cell death and impaired nervous system function. There is also a connection to paraneoplastic neurological syndromes where the autoimmune response against HuC/D proteins among others leads to neuronal damage. The relationship between HuD and proteins such as TAU becomes relevant here as disruptions in HuD function can impact TAU pathology critical in neurodegenerative diseases.