Abcam, ab210575, Mouse/Rat Cytochrome C ELISA Kit

Size: 1 x 96Tests
Mouse/Rat Cytochrome C ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat Cytochrome C with a sensitivity of 44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts,
Reacts with:Mouse, Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 44 pg/mL,
Range:487.4 - 14000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse/Rat Cytochrome C ELISA Kit (ab210575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytochrome C protein in tissue extracts, cell culture media, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat Cytochrome C with 44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cytochrome C is 11 kDa mitochondrial intermembrane space electron carrier protein. The oxidized form of the cytochrome C heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome C then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Cytochrome C also plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial outer membrane permeability resulting in release of cytochrome C into the cytosol. Binding of cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.
Biological function summary
Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.
Pathways
Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.
Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.