Abcam, ab210966, Human Pro-Collagen I alpha 1 ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human Pro-Collagen I alpha 1 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human pro-collagen I alpha 1 with a sensitivity of 5.3 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA – 450 nm readout, compatible with any standard plate reader - Available in different formats – 384-well format available for high-throughput applications - Broad sample compatibility – works with serum, plasma, cell culture extracts, tissue extracts, and cell culture supernatant - Cited in over 40 publications
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.3 pg/mL,
Range:39.06 - 2500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Pro-Collagen I alpha 1 ELISA Kit ab210966 is a rapid single-wash 90-min ELISA kit to measure Human Pro-Collagen I alpha 1 in cell culture extracts, tissue extracts, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 5.3 pg/mL.
How the assay works
Human Pro-Collagen I alpha 1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Pro-Collagen I alpha 1 protein in serum, plasma, cell culture supernatant, and cell and tissue extract samples only.
Human Pro-Collagen I alpha 1 ELISA Kit ab210966 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Pro-Collagen I alpha 1/ COL1A1 ELISA kit ab210966
Human Pro-Collagen I alpha 1/ COL1A1 ELISA kit ab210966 has been used to study cartilage regeneration and osteoarthritis treatment
and the effects of cigarette smoke and the sun on skin aging
References:
Kerong Dai et al. 2022, PMID: 36114225,
Roxane Pouliot et al. 2023, PMID: 37863977
Save your precious samples
Our 384-well Human Pro-Collagen I alpha 1 ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free
ab241825
- Anti-Procollagen Type 1 antibody [EPR19126-161] - BSA and Azide free (Capture)
ab242540
- Anti-Procollagen Type 1 antibody [EPR19126-152] - BSA and Azide free (Detector)
ab242788
Related and recommended products
Human Pro-Collagen I alpha 1/COL1A1 ELISA kit ab210966 is commonly used to study tissue structure and function. Human Pro-Collagen 1 alpha 1 is a component of the extracellular matrix (ECM).
Other ELISA kits to measure the ECM and its role in tissue function include:
- Human Fibronectin ELISA kit
ab219046
- Human MMP1 ELISA kit
ab215083
- Human Elastin ELISA kit
ab239433
The antibodies in this kit are generated against Pro-Collagen I N-Terminal Propeptide (PINP). Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, human Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-161), the mature chain (aa 162-1218), and another propeptide (aa 1219 – 1464). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C