Abcam, ab210968, Human VE Cadherin ELISA Kit

Size: 1 x 96Tests
Human VE Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human VE Cadherin with a sensitivity of 331 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 331 pg/mL,
Range:0.63 - 40 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human VE Cadherin ELISA Kit (ab210968) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VE Cadherin protein in cell culture supernatant, plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VE Cadherin with 331 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
VE-Cadherin (also known as Cadherin 5 or CD144) is a calcium dependent cell-cell adhesion glycoprotein. VE-Cadherin is a member of the cadherin superfamily, which interact with themselves in connecting cells. VE-Cadherin is expressed in endothelial cells and has role establishing and maintaining endothelial polarity and intercellular junctions.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.
Biological function summary
VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.
Pathways
VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.
VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.