Abcam, ab211313, Recombinant human NME2 protein

Size: 20µg
Recombinant human NME2 protein is a Human Fragment protein, in the 1 to 152 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncS, SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity is >1,800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25°C in a couple system with PK/LDH.,
Accession:P22392,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NME2 also known as NM23-H2 is a nucleoside diphosphate kinase that is involved in the synthesis of nucleoside triphosphates. With a mass of approximately 17 kDa it plays a role in the energetic metabolism of cells. NME2 is widely expressed in various tissues including the liver heart and brain. Its expression levels indicate its importance in maintaining regular cellular functions.
Biological function summary
NME2 participates in the regulation of transcription and cellular differentiation. It acts within the context of protein complexes which influence DNA-binding activities required for transcriptional control. NME2 also has roles in the regulation of cell proliferation differentiation and signaling. These activities demonstrate the protein's significance in maintaining normal physiological processes.
Pathways
NME2 is active in the MAPK/ERK signaling pathway significant in cell growth and differentiation. It interacts with other proteins like ERK2 to modulate downstream signaling cascades. This protein also features in the GTP nucleotide biosynthesis pathway where it cooperates with other kinases for nucleotide metabolism highlighting its important role in cell cycle regulation.
Mutations or altered expression of NME2 link with various cancers including breast and colon cancer. This connection emphasizes its potential role as a biomarker or therapeutic target. The protein also associates with other oncogenes like NME1 together contributing to the metastatic potential of cancer cells. NME2's involvement in chronic conditions like cancer confirms its importance in pathological states where it may influence disease progression or serve as a clinical marker.