Abcam, ab211488, Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal N2,N2-dimethylguanosine (m2,2G) antibody. Suitable for Dot, IP, ELISA and reacts with Modified Nucleic Acid samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR-19838-40,
Isotype:IgG,
Carrier free:No,
Applications:IP, ELISA, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Has been developed to discriminate between the modified base N2,N2-dimethylguanosine (m2,2G) and the unmodified counterpart Guanosine (G).

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N2N2-dimethylguanosine (m22G) is a modified nucleoside found in various types of RNA including tRNA and rRNA. It plays a role in the cellular machinery by contributing to the structural stability and function of RNA molecules. Its mass is approximately 297 g/mol. Expression of m22G occurs in the cellular RNA of many organisms reflecting its essential role in RNA structure. The modification m22G often referred to as a methylated derivative of guanosine is implicated in numerous cellular processes due to its presence in key RNA components.
Biological function summary
The modification of guanine at the N2 position influences RNA stability and function by enhancing the efficiency of translation. It forms part of the ribosome's active sites appearing as part of larger ribonucleoprotein complexes which are important for carrying out protein synthesis. This modification helps maintain the correct structure of ribosomal RNA ensuring accurate and efficient protein production. The presence of m22G in RNA components aids in the prevention of frameshift mutations during translation marking its importance in maintaining genetic fidelity.
Pathways
M22G plays a significant role in the translation and protein synthesis pathway contributing to the proper assembly and function of ribosomes. The presence of m22G within these pathways ensures the proper execution of genetic code translation interacting closely with proteins like ribosomal protein S15 and other translational machinery components. These interactions are essential in coordinating the synthesis of proteins impacting cellular growth and development functions.
M22G has connections with certain cancers and neurological disorders due to its involvement in regulating RNA stability and translation efficiency. Alterations or dysregulation in the patterns of m22G methylation can contribute to carcinogenesis by affecting the accuracy of protein translation. Proteins involved in this dysregulation include ribosomal proteins and RNA methyltransferases which can lead to the development of malignant transformation. Additionally insufficient or aberrant methylation of guanosine can contribute to neurodegenerative diseases like Alzheimer's where impaired RNA function results from transcriptional and translational inefficiencies.