Abcam, ab212165, Human Lipoprotein A ELISA Kit

Size: 1 x 96Tests
Human Lipoprotein A ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human lipoprotein A with a sensitivity of 2.5 ng/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader - Broad sample compatibility – suitable for serum, plasma, urine, and cerebrospinal fluid - Cited in 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.5 ng/mL,
Range:17.2 - 1100 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Lipoprotein A ELISA Kit ab212165 is a rapid single-wash 90-min ELISA kit to measure Human Lipoprotein A in cerebral spinal fluid, urine, serum and plasma. This SimpleStep ELISA® sensitivity is 2.5 ng/mL.
How the assay works
Human Lipoprotein A SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Lipoprotein A protein in serum, plasmas, urine, and cerebrospinal fluid samples only. Cellular and tissue extract samples have not been tested with this kit.
Human Lipoprotein A ELISA Kit ab212165 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Lipoprotein A ELISA Kit ab212165
Human Lipoprotein A ELISA Kit ab212165 has been used to study the effects of LDL binding in obesity after bariatric surgery
and Lipoprotein A as a predictor of coronary events
References:
Olga Gurskyi et al. 2023, PMID: 37777014,
Elena Aikawa et al. 2022, PMID: 35724702
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Lipoprotein A Antibody Pair - BSA and Azide free
ab241862
- Anti-Lipoprotein a antibody [EPR19731-148] - BSA and Azide free (Capture)
ab242565
- Anti-Lipoprotein a antibody [EPR19731-165] - BSA and Azide free (Detector)
ab242813
Related and recommended products
Human Lipoprotein A ELISA Kit ab212165 is commonly used to study cardiovascular diseases and lipid metabolism
Other ELISA kits to measure lipid metabolism are:
- Human Apoliporotein B ELISA kit
ab190806
Lipoprotein A is an atherogenic lipoprotein particle formed by an assembly of LDL particles and apo(a) bound to apoB-100 component of LDL. Apolipoprotein A, the main constituent of Lipoprotein A, has serine proteinase activity and is capable of autoproteolysis. Apolipoprotein A has 4,548 amino acids, variable sizes from 200 to 700 kDa, multiple isoforms, and structural homology with plasminogen. It competes with plasminogen for its binding site, inhibiting tissue-type plasminogen activator 1 and leading to reduced fibrinolysis. High levels of Lipoprotein A in the blood is a risk factor for myocardial infarction (MI), coronary heart disease (CHD), cerebrovascular disease (CVD), atherosclerosis, thrombosis, and stroke.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lipoprotein a also known as Lp(a) or LPA is a lipoprotein particle in the bloodstream characterized by the binding of apolipoprotein(a) to apolipoprotein B-100 on a low-density lipoprotein (LDL) core. It has a molecular weight that varies depending on the size of apolipoprotein(a) typically ranging from 300 to 700 kDa. This protein is primarily synthesized in the liver and its levels are mostly determined by genetic factors. Lp(a) has structural similarity to LDL and plasminogen each playing distinct roles in the body.
Biological function summary
Lp(a) is involved in lipid metabolism and occasionally plays a role in the modulation of fibrinolysis due to its structural resemblance to plasminogen. This resemblance allows it to competitively inhibit plasminogen activation which may influence clot stabilization and breakdown. Lp(a) does not appear to form part of a larger functional complex but can affect processes where plasminogen is active. It plays a dual role in both lipid transport and coagulation processes.
Pathways
The role of Lp(a) is significant in the cardiovascular system particularly in the atherosclerosis pathway. It interacts mainly through its structural mimicry of LDL and plasminogen leading to competitive interactions with these molecules. Lp(a)'s impact on the pathway involves promoting the formation of atherosclerotic plaques by depositing within arterial walls and contributing to pro-inflammatory activities. The primary proteins related in this context include LDL components and plasminogen.
Lp(a) levels above 200 nanomoles per liter are associated with an increased risk of cardiovascular diseases especially atherosclerosis and myocardial infarction. These elevated levels can lead to enhanced plaque formation in the arteries due to Lp(a)'s affinity for binding to phospholipids and arterial wall components. Furthermore it shares pathway associations with apolipoprotein B-100 linking it to familial hypercholesterolemia where abnormalities in lipid metabolism are evident. Both genetic and environmental factors contribute to the variation in Lp(a) levels and its pathological implications in these conditions.