Abcam, ab212166, Human BDNF ELISA Kit

Size: 1 x 96Tests / 10 x 96Tests
Human BDNF ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify BDNF with a sensitivity of 2.4 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric assay – 450 nm readout, compatible with any standard plate reader - Available in different formats – 384-well format available for high-throughput applications- Broad sample compatibility – works with serum, plasma, cell culture supernatants, and cerebrospinal fluid- Cited in over 30 publications
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.4 pg/mL,
Range:15.6 - 1000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human BDNF ELISA Kit ab212166 is a rapid single-wash 90-min ELISA kit to measure Human BDNF in cerebral spinal fluid, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 2.4 pg/mL.
How the assay works
Human BDNF SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human BDNF protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
Human BDNF ELISA Kit ab212166 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human BDNF ELISA Kit ab212166
Human BDNF ELISA Kit ab212166 has been used in publications to study intermittent hypoxia training as a prevention for learning-memory decline in Alzheimer's Disease
, the association between BDNF levels and postural instability and gait disturbance in Parkinson's Disease
, and the effects of alcohol on BDNF blood levels
References:
Xiangrong Shi et al. 2021 PMID: 34276338,
Chien Tai Hong et al. 2021 PMID: 32932791,
Amirhesam Alirezaei et al. 2023 PMID: 37845289
Related and recommended products
Human BDNF ELISA Kit ab212166 is commonly used to study neural health.
Other kits for measuring the survival, growth, and differentiation of neurons include:
- Human Agrin ELISA Kit
ab216945
- Human IL-6 ELISA kit
ab178013

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C