Abcam, ab213760, Human CD5L / CT-2 ELISA Kit

Size: 1 x 96Tests
Human CD5L / CT-2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD5L / CT-2 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:156 - 10000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human CD5L / CT-2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213760) is designed for the quantitative measurement of Human CD5L / CT-2 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD5L / CT-2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S20-G347) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD5L / CT-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD5L / CT-2 amount of sample captured in plate.
CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence
in situ
hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis
in vitr
o and undergo accelerated apoptosis in atherosclerotic lesions
in vivo
. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD5L also known as CT-2 or CD5 antigen-like is a secreted glycoprotein with a molecular mass of approximately 50 kDa. This protein expresses mainly in liver and to a lesser extent in spleen lymph nodes and other immune-related tissues. The CD5L protein acts as a scavenger receptor cysteine-rich (SRCR) superfamily member which participates in pathogen recognition and clearance. Researchers often measure CD5L levels using CD5L ELISA kits providing quantitative analysis of this biomarker in various biological samples.
Biological function summary
CD5L influences several immune processes modulating macrophage function and lipid metabolism. It plays a role within a complex system transforming macrophages into an anti-inflammatory phenotype. This helps the clearance of apoptotic cells and prevents tissue damage during inflammation. CD5L also impacts cholesterol homeostasis contributing to lipid clearance and influencing foam cell formation which serves a significant role in atherosclerosis development.
Pathways
This protein integrates into the lipid metabolism pathway and the immune regulation pathway. Within the lipid metabolism pathway interaction with proteins such as apolipoprotein AI demonstrates its role in controlling cholesterol and triglyceride levels. In the immune regulation pathway CD5L collaborates with other immune-related proteins including CD36 and TLR2 to regulate immune response and inflammation affecting macrophage activation and phagocytosis.
CD5L associates with atherosclerosis and systemic lupus erythematosus (SLE). Researchers link elevated CD5L levels with atherosclerosis due to its ability to drive macrophage transformation into foam cells linking it with cardiovascular diseases. In systemic lupus erythematosus CD5L interacts with protein serum amyloid A (SAA) exacerbating the inflammatory processes associated with autoimmune disorders. Understanding CD5L's role may provide insights for developing therapeutic strategies for these conditions.