Abcam, ab213763, Human CD97 ELISA Kit

Size: 1 x 96Tests
Human CD97 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD97 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:125 - 8000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human CD97 Enzyme-Linked Immunosorbent Assay (ELISA) Kit (ab213763) is designed for the quantitative measurement of Human CD97 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD97 has been pre-coated onto 96-well plates. Standards (NSO, Q21-Q398) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD97 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD97 amount of sample captured in plate.
Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence
in situ
hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD97 also known as adhesion G protein-coupled receptor E5 is a member of the EGF-TM7 subfamily of adhesion GPCRs. The protein has a mass of approximately 83-93 kDa and consists of distinct extracellular transmembrane and cytoplasmic domains. CD97 expresses highly on cells of the immune system especially granulocytes activated T-lymphocytes and smooth muscle cells. Researchers frequently link CD97 expression to immune cell activation and migration processes.
Biological function summary
CD97 plays a significant role in cell communication and adhesion. The protein is part of a multiprotein complex and interacts with chondroitin sulfate and other heparan sulfate proteoglycans. This interaction modulates inflammatory responses and tissue remodeling. CD97 influences immune responses by mediating cellular interaction and signaling through its long extracellular domain. Its activity is essential for leukocyte trafficking and extravasation.
Pathways
CD97 participates in important cell adhesion and immune system pathways. It interacts notably with the EGF-like crossover domains and the GPCR pathways. Related proteins such as CD55 also known as decay-accelerating factor interact with CD97 influencing the immune regulatory processes. These interactions enhance our understanding of leukocyte recruitment and the maintenance of tissue architecture during inflammatory responses.
CD97 is associated with autoimmune diseases and cancer. High CD97 expression levels relate to diseases like rheumatoid arthritis and certain types of tumors including gastric carcinoma. In cancer CD97 interacts with integrin molecules promoting metastasis and tumor invasion. Understanding CD97's connection to integrins and other components could provide insights into therapeutic targets for autoimmune and cancer treatment.