Abcam, ab214025, Human IL-1 beta ELISA Kit

Size: 1 x 96Tests / 10 x 96Tests
Human IL-1 beta ELISA kit is a high-sensitivity SimpleStep ELISA® assay designed for the quantification of human interleukin-1 beta (IL-1β) in with a sensitivity of 5.64 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric Sandwich ELISA - 450nm readout; works on any plate reader - Different formats for different needs: 10x96 plates for bulk orders - Cited in over 90 publications
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.64 pg/mL,
Range:14.06 - 900 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IL-1 beta ELISA Kit ab214025 is a rapid single-wash 90-min ELISA kit to measure Human ALT in cell culture supernatant, serum, plasma. This SimpleStep ELISA® sensitivity is 5.64 pg/mL.
How the assay works
Human ALT SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human ALT protein in serum, plasma, and cell culture supernatant samples only. Tissue extract samples have not been tested with this kit. This kit is incompatible with plasma- heparin and cell extract samples.
Human IL-1 beta ELISA Kit ab214025 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IL-1 beta ELISA Kit ab214025
Human IL-1 beta ELISA Kit ab214025 has been used to study inflammation in various physio-pathological contexts such as pneumonia
, osteoarthritis
, and diabetes
References:
Jinxing Hu et al. 2023, PMID: 38023363,
Zhong Wu et al. 2023, PMID: 37842584,
Raffaele Marfella, et al. 2023, PMID: 37005586
Save your precious samples
Our 384-well Human IL-1 beta ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Monkey IL-1beta Antibody Pair - BSA and Azide free
ab241807
- Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture)
ab242561
- Anti-IL-1 beta antibody [EPR18815-128] - BSA and Azide free (Detector)
ab242986
Related and recommended products
Human IL-1 beta ELISA Kit ab214025 is commonly used to measure IL-1 beta as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human IL-6 ELISA kit
ab178013
- Human TNF alpha ELISA kit
ab181421
- Human IL-18 ELISA kit
ab215539
To measure IFN gamma at a lower dynamic range (12.5-1600 pg/mL):
- Human IFN gamma High Sensitivity ELISA kit
ab236895
Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C