Abcam, ab214030, Human IL-8 ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Human IL-8 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-8 (IL-8) with a sensitivity of 1.8 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader - Available in different formats to meet various throughput needs, including 384-well plates for higher throughput - Broad sample compatibility – suitable for serum, plasma, cell culture supernatants, and cerebrospinal fluid - Cited in over 30 publications
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.8 pg/mL,
Range:3.91 - 250 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IL-8 ELISA Kit ab214030 is a rapid single-wash 90-min ELISA kit to measure Human IL-8 in cerebral spinal fluid, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 1.8 pg/mL.
How the assay works
Human IL-8 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human IL-8 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
Human IL-8 ELISA Kit ab214030 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IL-8 ELISA Kit ab214030
Human IL-8 ELISA Kit ab214030 has been used to assess inflammation in different physio-pathological contexts such as traumatic brain injury
, pulmonary disease
, and osteoarthritis
References:
Jindan Gao et al. 2022, PMID: 36247290,
Rui Zheng et al. 2022, PMID: 35191419,
Zhi Lv et al. 2021, PMID: 34082824
Save your precious samples
Our 384-well Human IL-8 ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Monkey IL-8 Antibody Pair - BSA and Azide free
ab241839
- Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture)
ab242515
- Anti-IL-8 antibody [EPR19358-108] - BSA and Azide free (Detector)
ab242983
Related and recommended products
Human IL-8 ELISA Kit ab214030 is commonly used to measure IL-8 as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA kit
ab181421
- Human IL-6 ELISA kit
ab178013
- Human IL-1 beta ELISA kit
ab214025
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C