Abcam, ab214039, Anti-Transferrin Receptor antibody [EPR20584]

Size: 20µL / 100µL / 1mL
Anti-Transferrin Receptor antibody [EPR20584] (ab214039) is a rabbit monoclonal antibody detecting Transferrin Receptor in Western Blot, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20584,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human, Rat,
Applications:IHC-P, IP, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Transferrin Receptor antibody [EPR20584] (ab214039) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP and WB.
Anti-Transferrin Receptor antibody [EPR20584] (ab214039) was first used in a scientific publication in 2019 and has been cited over 52 times in peer reviewed journals. It's performance in Western blot in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Transferrin Receptor antibody [EPR20584] (ab214039) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Transferrin Receptor antibody [EPR20584] (ab214039) specifically detects Transferrin Receptor (UniProt ID: P02786; Molecular weight: 85kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR20584 -
ab232376
Antibody clone EPR20584 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
ab317529
The transferrin receptor, crucial for cellular iron uptake, is often overexpressed in cancer cells, making it a valuable target for antibody-mediated therapies. Its role in promoting tumor growth and survival highlights its potential as a therapeutic target and a prognostic marker in oncology.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.
Biological function summary
TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.
Pathways
The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.
Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.