Abcam, ab214586, Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free

Size: 100µg / 1mg
Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation. Suitable for Western Blot, Flow Cytometry, IHC-P, ICC/IF in Human, Mouse, Rat . - Clone EP1601Y is the most cited clone to Cytokeratin 5 - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1601Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Mouse reactivity is based on IHC (positive tissues: Liver, lung, brain and skin). However, WB was negative for Mouse brain, heart, kidney and spleen. There is background staining in mouse and rat islet.

Product details:
What is this antibody validated in?
Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Cytokeratin 5?
Anti-Cytokeratin 5 [EP1601Y] - BSA and Azide free (ab214586) specifically detects a band for Cytokeratin 5 (UniProt: P13647) at a molecular weight of 62kDa.
Trusted by the scientific community
Anti-Cytokeratin 5 [EP1601Y] - BSA and Azide free (ab214586) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EP1601Y] also available for your convenience:
ab52635
, Alexa Fluor® 488 -
ab193894
, Alexa Fluor® 647 -
ab193895
, HRP -
ab193896
, Carrier free - ab214586, APC -
ab224984
, PE -
ab224985
ab321868
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze