Abcam, ab215539, Human IL-18 ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human IL-18 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-18 with a sensitivity of 8.3 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 40 citations
Key facts
Detection method:Colorimetric,
Sample types:Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.3 pg/mL,
Range:62.5 - 4000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IL-18 ELISA kit ab215539 is a rapid single-wash 90-min sandwich ELISA kit to measure Human IL-18 in Urine, Cell culture extracts, Tissue Extracts, Plasma (Citrate, EDTA, Heparin) Cell culture supernatant, and Serum. This Simplestep sensitivity is 8.3 pg/mL.
How the assay works
Human IL-18 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Human IL-18 ELISA kit ab215539 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
How other researchers are using Human IL-18 ELISA kit ab215539
Human IL-18 ELISA kit ab215539 has been used to study melanoma
, obesity
, and age-related diseases
References:
Y. Zhang, et.al., 2023, PMID: 37620327
S. Geng, et.al., 2023, PMID: 38156278
B. Chhunchha, et.al., 2023, PMID: 38003466
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (Capture)
ab242590
Anti-IL-18 antibody [EPR19956-19] - BSA and Azide free (Detector)
ab242856
Related and Recommended products
Human IL-18 ELISA Kit ab215539 is commonly used to measure IL-18 as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human IL-18BP ELISA Kit
ab224877
- Human IL-37 ELISA Kit
ab300313
- Human IRAK1 ELISA Kit
ab219630
- Human IRAK4 ELISA Kit
ab213472
Interleukin-18 (IL-18 or Interferon gamma-inducing factor) is a proinflammatory cytokine in the IL-1 superfamily. IL-18 has overlapping and synergistic biological function with IL-12. IL-18 is found in both intracellular and extracellular compartments. The antibodies in this kit were raised to the mature full-length sequence of human IL-18. Human IL-18 has 66% protein sequence identity to mouse Lipocalin-2.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C