Abcam, ab216071, Anti-AGR2 + AGR3 antibody [EPR20164-222]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Anterior Gradient 2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20164-222,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AGR2 (anterior gradient protein 2) and AGR3 (anterior gradient protein 3) are small proteins with roles in cellular homeostasis. AGR2 has a mass of approximately 20 kDa while AGR3 is around 18 kDa. Both proteins localize in the endoplasmic reticulum (ER) and are expressed notably in epithelial tissues like the gastrointestinal tract and respiratory epithelium. AGR2 also known as PDIA17 functions as a protein disulfide isomerase which assists in protein folding. AGR3 shares structural similarities with AGR2 suggesting overlapping functions.
Biological function summary
AGR2 and AGR3 influence processes such as cell growth and repair. They are necessary for maintaining the proper folding of secreted and membrane proteins. AGR2 interacts with mucin proteins and this complex formation is important for mucin production which supports protective mucus barriers. AGR3's specific biological partners are less well-defined but it complements AGR2's functions in similar tissue contexts suggesting it might share similar interactions.
Pathways
AGR2 and AGR3 engage in pathways that link ER function to broader cellular activities. One important pathway involves the unfolded protein response (UPR) where AGR2 helps mitigate ER stress by ensuring proper protein assembly and folding. Additionally AGR2 participates in the ERK signaling pathway impacting cell proliferation and differentiation. Proteins such as ATF6 and GRP78 interact with AGR2 in these pathways indicating its role in stress response and growth signaling networks.
AGR2 and AGR3 gain attention in relation to cancer and cystic fibrosis. In cancer especially breast and prostate AGR2 overexpression correlates with tumor progression and metastasis. It interacts with proteins like MUC1 and HER2 facilitating oncogenic pathways. AGR3 while less studied shows similar trends suggesting potential involvement. In cystic fibrosis AGR2 associates with compromised mucin production and secretion causing exacerbated lung conditions. CXCL8 a mucin-related protein is seen alongside AGR2 activity in these inflammatory scenarios.