Abcam, ab216463, Anti-VGLUT2 antibody [EPR21085]

Size: 100µL / 1mL
Anti-VGLUT2 antibody [EPR21085] (ab216463) is a rabbit monoclonal antibody detecting VGLUT2 in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF . Suitable for Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21085,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IP, WB, IHC-P, ICC/IF, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-VGLUT2 antibody [EPR21085] (ab216463) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse, Rat samples.
What is the molecular weight of VGLUT2?
Anti-VGLUT2 [EPR21085] (ab216463) specifically detects a band for VGLUT2 (UniProt: Q8BLE7) at a molecular weight of 64kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR21085] also available for your convenience: ab216463, Carrier free -
ab227906
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ab310989
, Alexa Fluor® 647 -
ab311115
, Alexa Fluor® 594 -
ab311736
, Alexa Fluor® 568 -
ab313014
, Alexa Fluor® 555 -
ab313218
, Alexa Fluor® 750 -
ab321388
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VGLUT2 also known as Vesicular Glutamate Transporter 2 or SLC17A6 plays a significant role in neurotransmission. This protein facilitates the transport of glutamate an excitatory neurotransmitter into synaptic vesicles in preparation for synaptic release. VGLUT2 has a molecular weight of approximately 61 kDa. Its expression appears particularly in the central nervous system including regions like the thalamus brainstem and some parts of the cerebral cortex.
Biological function summary
VGLUT2 is essential for proper glutamatergic signaling in neuronal communication. The protein does not form part of a larger complex but functions individually to maintain synaptic vesicle filling with glutamate. Normal glutamatergic synaptic transmission relies on VGLUT2's ability to concentrate glutamate in synaptic vesicles. This concentration is necessary for effective excitatory signaling impacting processes such as synaptic plasticity learning and memory.
Pathways
VGLUT2 serves a critical function in neurotransmitter release and synaptic vesicle cycle. The protein actively transports glutamate into synaptic vesicles enabling its release into the synaptic cleft. VGLUT2 interacts closely with proteins like SNAREs that assist in vesicle docking and fusion. The protein also plays a functional role in the glutamatergic pathway which is important for excitatory neurotransmission in the brain.
VGLUT2 holds relevance to conditions such as schizophrenia and epilepsy. Its dysregulation can alter glutamate levels potentially contributing to altered neuronal excitability and synaptic dysfunction. Studies link VGLUT2 with connected proteins like NMDA receptors which mediate excitatory postsynaptic potentials and are associated with glutamatergic signaling disruptions in these disorders. Alterations in the expression or function of VGLUT2 might influence susceptibility to these neuropsychiatric and neurological conditions.