Abcam, ab216839, Anti-ATF-4 antibody

Size: 100µL
Anti-ATF-4 antibody (ab216839) is a rabbit polyclonal antibody detecting ATF-4 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 10 publications
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ATF4 aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P18848

Product details:
What is this antibody validated in?
Anti-ATF-4 antibody (ab216839) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of ATF-4?
Anti-ATF-4 (ab216839) specifically detects a band for ATF-4 (UniProt: P18848) at a molecular weight of 38kDa.
Trusted by the scientific community
Anti-ATF-4 (ab216839) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Activating Transcription Factor 4 (ATF-4) also known as CREB-2 is a fundamental protein involved in cellular stress responses. It functions mechanically as a transcription factor that regulates gene expression in reaction to stress signals. The molecular weight of ATF-4 is approximately 38 kDa. ATF-4 gets expressed in various tissues including the brain liver and pancreas reflecting its involvement in diverse cellular processes. Scientists widely use techniques like Western blot to detect and study ATF-4 expression patterns due to its reliable measurement of the ATF-4 molecular weight.
Biological function summary
ATF-4 participates in controlling genes linked to amino acid metabolism redox homeostasis and apoptosis. It does not work alone; ATF-4 often forms part of larger complexes interacting with other transcription factors like C/EBP and ATF-3 to exert its effects. These interactions enable it to respond accurately to different types of cellular stress by adjusting the expression of specific genes ensuring that cells can adapt to changing conditions.
Pathways
ATF-4 plays a significant role in the integrated stress response (ISR) and the unfolded protein response (UPR). Through these pathways it collaborates with proteins such as PERK (protein kinase R-like endoplasmic reticulum kinase) and eIF2α. The ISR and UPR help cells cope with stress by modulating protein synthesis and promoting the expression of protective genes. By interacting with these pathways ATF-4 contributes to maintaining cellular homeostasis and protecting cells from damage.
ATF-4 has been linked to conditions such as neurodegenerative diseases and cancer. In neurodegenerative disorders like Alzheimer's disease ATF-4 can regulate genes involved in neuronal survival and apoptosis interacting with proteins like CHOP. In cancer ATF-4 influences tumor cell survival and growth through its role in stress responses. Understanding ATF-4's relationship with diseases highlights its potential as a therapeutic target in the treatment of these complex disorders.