Abcam, ab217068, Anti-MPP5/Pals1 antibody

Size: 100µg
Rabbit Polyclonal MPP5/Pals1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PALS1 aa 600-650.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PALS1 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.Q8N3R9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab217068 was affinity purified using an epitope specific to MPP5/Pals1 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MPP5 also known as Pals1 is a membrane-associated guanylate kinase (MAGUK) protein. It has a molecular weight of approximately 75 kDa. MPP5 is expressed in various tissues with significant levels in the brain retina and epithelial cells. MPP5 is important in cell junction organization ensuring proper cellular architecture and polarity. It occupies a role as a scaffolding protein forming large protein complexes at cell membranes.
Biological function summary
MPP5 is part of the Crumbs protein complex which includes Crb3 and PatJ proteins. This complex is essential for maintaining apical-basal polarity in epithelial cells. MPP5 contributes to cell polarity and adhesion ensuring integrity and proper functioning of epithelial tissue. Disruptions in this protein complex can lead to loss of cell polarity affecting tissue organization and stability.
Pathways
MPP5 interacts with proteins in the Hippo signaling pathway which controls organ size and cell growth. MPP5 influences the activity of YAP (Yes-associated protein) a critical component in the Hippo pathway. YAP regulation by MPP5 can affect cellular proliferation and apoptosis linking MPP5 to tissue growth and regeneration processes.
Mutations or dysregulation in MPP5 have connections to retinal degeneration and epithelial cancers. Its interaction with proteins like CRB1 in the retina influences photoreceptor development and retinal maintenance. Abnormal expression of MPP5 can lead to disrupted cell polarity contributing to cancer progression and tumor metastasis in epithelial tissues. Understanding MPP5's role in these diseases helps in identifying potential therapeutic targets.