Abcam, ab2174, Anti-Huntingtin (phospho S421) antibody

Size: 100µg
Rabbit Polyclonal Huntingtin phospho S421 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HTT phospho S421 aa 400-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human HTT phospho S421 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.P42858,
Specificity:Detects a 190kDa band in PC-3 cells, which may correspond to Huntingtin. Stains neuronal cytoplasm in immunohistochemistry (the expected localisation). The antibody has not been conclusively tested for specificity for phospho S421 Huntingtin.This antibody is specific for phosphorylated human Huntington protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntington homologues from chimpanzee, pig and chicken. Cross reactivity with Huntington protein homologues from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Anti-Huntingtin pS421 is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Huntington protein at the pS421 residue., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle