Abcam, ab218095, Recombinant human/mouse Ihh protein

Size: 5µg / 25µg / 100µg
Recombinant human/mouse Ihh protein is a Human Fragment protein, in the 29 to 202 aa range, expressed in Escherichia coli, with 97%, < 0.05 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.
Key facts
Purity:97% SDS-PAGE,
Endotoxin level:< 0.05 EU/µg,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:HPLC, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q14623,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute at 0.1 mg/mL in 10 mM HCl,
Storage buffer:Constituents: 0.1% Trifluoroacetic acid

Product details:
This product shares 100% homology with amino acids 29 -202 of Mouse IHH.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Indian hedgehog (Ihh) protein known by its gene symbol IHH plays key roles in cell signaling. Ihh with an approximate mass of 45 kDa belongs to the Hedgehog family of signaling molecules. It is expressed in various tissues including cartilage bone and parts of the gastrointestinal tract where it regulates growth and patterning. Ihh acts by binding to its receptor Patched triggering downstream signaling that influences cell fate decisions.
Biological function summary
Ihh is significant in regulating the differentiation and proliferation of chondrocytes in the growth plate of long bones. It acts as part of a complex regulatory network coordinating with Parathyroid hormone-related protein (PTHrP) to maintain proper development of endochondral bones. Ihh also influences epithelial and mesenchymal interactions during embryo development evidencing its important role in developmental biology.
Pathways
Ihh is a critical component of the Hedgehog signaling pathway a conserved pathway involved in embryonic development and adult tissue homeostasis. This pathway also includes proteins like Sonic hedgehog (Shh) and Desert hedgehog (Dhh) as well as the receptor Smoothened which mediates the signaling cascade. Ihh signaling impacts pathways such as the Wnt pathway influencing the proliferation and differentiation of cells thereby highlighting its integration into broader cellular signaling networks.
Ihh misregulation can lead to skeletal disorders like brachydactyly type A1 characterized by shortened fingers and toes. Furthermore abnormal Ihh signaling can contribute to certain cancers such as chondrosarcoma where overactive Hedgehog signaling promotes tumorigenesis. The relationship between Ihh and other Hedgehog proteins including Shh is key in understanding these pathologies as they share pathways that are often disrupted in disease scenarios.