Abcam, ab219046, Human Fibronectin ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human Fibronectin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Fibrionectin with a sensitivity of 20.6 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human, Rhesus monkey,
Assay type:Sandwich (quantitative),
Sensitivity:= 20.6 pg/mL,
Range:125 - 8000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Fibronectin ELISA Kit ab219046 is a rapid single-wash 90-min ELISA kit to measure Human Fibronectin in cell culture extracts, tissue extracts, cell culture supernatant, milk, serum and plasma. This SimpleStep ELISA® sensitivity is 20.6 pg/mL.
How the assay works
Human Fibronectin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Fibronectin protein in serum, plasma (citrate and EDTA), milk, cell culture supernatant, and cell and tissue extract samples only.
Recombinant human Anastelin was prepared within the working range of the assay and assayed for cross reactivity. No cross-reactivity was observed.
Human Fibronectin ELISA Kit ab219046 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Fibronectin ELISA Kit ab219046
Human Fibronectin ELISA Kit ab219046 has been used to study wound healing after dermato-oncological surgery
and the association of NGAL and fibrosis in diabetic nephropathy
References:
Cristina Magnoni et al. 2023, PMID: 36836820,
Guiying Li et al. 2021, PMID: 33732294
Save your precious samples
Our 384-well Human Fibronectin ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Fibronectin Antibody Pair - BSA and Azide free
ab241837
- Anti-Fibronectin antibody [EPR19331-123] - BSA and Azide free (Capture)
ab242680
- Anti-Fibronectin antibody [EPR19331-44] - BSA and Azide free (Detector)
ab242925
Related and recommended products
Human Fibronectin ELISA Kit ab219046 is commonly used to study tissue structure and function. Fibronectin is a component of the extracellular matrix (ECM).
Other ELISA kits to measure the ECM and its role in tissue function include:
- Human Pro-Collagen I alpha 1 ELISA kit
ab210966
- Human alpha SMA ELISA Kit
ab240678
- Human MCP-1 ELISA Kit
ab179886
- Human Elastin ELISA kit
ab239433
Fibronectin is a large glycoprotein present in the extracellular matrix and circulating plasma. Fibronectin is important in many cell adhesion and migration related processes, including wound healing, embryogenesis and nerve regeneration. Differential expression of fibronectin is seen in coronary heart disease, glomerulopathy and tumor cell metastasis. The protein contains binding sites for collagen, heparin and fibrin and is a specific ligand for several integrin adhesion receptors. Fibronectin exists as a dimer or multimer.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C