Abcam, ab219800, Anti-GSDMD antibody [EPR20859]

Size: 20µL / 100µL / 1mL
Anti-GSDMD antibody [EPR20859] (ab219800) is a rabbit monoclonal antibody detecting GSDMD in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Mouse, Rat, Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 150 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20859,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, ICC/IF, WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody can detect full length, as well as a N-terminal fragment after stimulation in WB.Expression level of GSDMD in whole normal brain lysate is low or undetectable (PMID: 32671214, PMID: 34975487), we recommend loading higher amount of lysate or using lower antibody dilution.Please see documentation for further information on positive controls (Chinese version)

Product details:
Anti-GSDMD antibody [EPR20859] (ab219800) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), IHC-P, IP and WB.
Anti-GSDMD antibody [EPR20859] (ab219800) was first used in a scientific publication in 2019 and has been cited over 152 times in peer reviewed journals. It's performance in Western Blot in mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-GSDMD antibody [EPR20859] (ab219800) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-GSDMD antibody [EPR20859] (ab219800) has been confirmed by Western Blot testing in GSDMD knockout Mouse liver and mouse lung whole cell lysates cells.
Anti-GSDMD antibody [EPR20859] (ab219800) specifically detects GSDMD (UniProt ID: Q9D8T2; Molecular weight: 54kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR20859 -
ab239377
Antibody clone EPR20859 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, PE, Alexa Fluor
647, FITC (
ab246711
ab246713
ab319081
ab322296
Gasdermin D (GSDMD) is crucial in inflammasomes for executing pyroptosis and releasing pro-inflammatory cytokines. GSDMD is implicated in autoimmune diseases like IBD.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GSDMD also known as gasdermin D is a protein known for its role in pyroptosis a form of programmed cell death. Its molecular weight is approximately 53 kDa. Mechanically GSDMD operates by forming pores in cell membranes. These pores disrupt cellular homeostasis and eventually lead to cell lysis. GSDMD is mainly expressed in immune cells including macrophages and neutrophils. Researchers frequently use GSDMD Western blot and GSDMD ELISA for its detection and quantification in various studies.
Biological function summary
Gasdermin D functions in the execution of immune responses against infections. It acts as an effector molecule that participates directly in pyroptosis by disrupting mitochondrial membranes. GSDMD operates as part of a larger inflammasome complex initiated by inflammatory signals. The inflammasome activates inflammatory caspases that cleave GSDMD enabling its active form to execute pyroptosis. This process releases cytokines like IL-1β enhancing the inflammatory response.
Pathways
GSDMD is important in the pyroptosis pathway initiated by the inflammasome. This process involves Caspase-1 a protease responsible for cleaving pro-inflammatory cytokines and initiating pyroptosis. Another significant pathway includes NLRP3 inflammasome which acts upstream to activate Caspase-1 and subsequently GSDMD establishing the overall inflammatory response in the innate immune system. Through these pathways GSDMD interacts closely with proteins like IL-18 an essential inflammatory mediator.
Gasdermin D has links to inflammatory diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis the excessive activation of GSDMD leads to chronic joint inflammation mediated by activated immune cells. In sepsis over-activation of the pyroptosis pathway may cause severe systemic inflammation driven by GSDMD activity exacerbating cytokine release. Connections exist between GSDMD and other proteins such as Caspase-11 which can also initiate GSDMD cleavage independently and has roles in non-canonical inflammasome pathways influencing these conditions.