Abcam, ab220278, Anti-PCF11 antibody

Size: 100µL
Rabbit Polyclonal PCF11 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PCF11 aa 1300-1450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PCF11 aa 1300-1450. The exact immunogen used to generate this antibody is proprietary information.O94913

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PCF11 occasionally referred to as "Polypeptide Cleavage Factor 1" is an essential player in the transcription termination process. This protein serves as a component of the cleavage and polyadenylation specificity factor complex enabling the final stages of pre-mRNA synthesis. PCF11 has a molecular mass of approximately 63 kDa and is highly expressed in the nucleus where transcription occurs. It is found in many types of tissues indicating its wide-ranging roles in cellular biology.
Biological function summary
PCF11 influences the even regulation of gene expression by aiding in the removal of RNA polymerase II from DNA which helps in the proper conclusion of transcription. It works as part of a larger complex partnering with other factors to ensure that mRNA is properly released and processed which is important for functional mRNA maturation. These actions highlight its importance in RNA metabolism and stability.
Pathways
PCF11 is intricately involved in the transcriptional regulation pathway especially influencing the maturation of mRNA through interactions with RNA polymerase II. It closely coordinates its activities with proteins such as CTD phosphatase and SCAF8 directing the proper progression and termination of transcription. Additionally its involvement in RNA processing links it to the spliceosome-mediated mRNA decay pathway ensuring accurate gene expression control.
Mutations and disruptions in PCF11 expression are linked to neurological disorders and various carcinomas. PCF11 anomalies can lead to defective RNA metabolism affecting neural development and cancer proliferation. In cancer it has connections with overexpressed proteins such as MYC which promotes malignant growth through misregulated transcription termination similarly impacting neurological health when dysregulated.