Abcam, ab221138, Anti-PHOS/PDC antibody [EPR21913]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PHOS/PDC antibody. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21913,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, IP, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein target PHOS/PDC also known by alternate names is essential for cellular metabolism playing a role in glycolysis and energy production. PHOS/PDC has a molecular mass that varies based on its phosphorylation state. It is expressed in various tissues with high levels in muscle and liver cells. In these tissues it presents as an important regulator in converting pyruvate into acetyl-CoA a critical step in energy production.
Biological function summary
PHOS/PDC is part of the pyruvate dehydrogenase complex which is important for linking glycolysis with the citric acid cycle. This protein complex facilitates the transformation of pyruvate molecules into acetyl-CoA a substrate for the Krebs cycle which is fundamental in cellular respiration and energy extraction. PHOS/PDC directly affects energy homeostasis in cells and subsequently impacts overall metabolic rate.
Pathways
PHOS/PDC functions integrally within the glycolysis and citric acid cycle pathways. It links glycolysis with the mitochondrial Krebs cycle by converting pyruvate into acetyl-CoA a pivotal step in the energy production and metabolic pathway. It associates closely with proteins such as acetyl-CoA synthetase and citrate synthase which further act within the citric acid cycle. Effective PHOS/PDC activity ensures efficient energy generation and metabolic integration.
PHOS/PDC malfunctions relate to metabolic conditions like pyruvate dehydrogenase deficiency and lactic acidosis. Disruptions in PHOS/PDC can impair energy production and lead to these conditions reflecting its importance in cellular metabolism. Additionally its activity connects to disorders involving other proteins like lactate dehydrogenase which can exacerbate metabolic dysfunctions when PHOS/PDC is impaired. Understanding these connections aids in the diagnosis and treatment of related metabolic disorders.