Abcam, ab222098, Anti-MICA + MICB antibody [EPR22071-91]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal MICA antibody. Suitable for IP, WB and reacts with Human, Recombinant fragment samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22071-91,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MICA and MICB also referred to as Mhc class I polypeptide-related sequence A and B are stress-induced proteins and are known to have a molecular weight of approximately 62 kDa and 70 kDa respectively. They are expressed on the surface of many cell types especially epithelial cells fibroblasts and upon certain cancers. These proteins are encoded within the MHC class I region yet unlike classic MHC class I molecules they do not associate with beta-2-microglobulin and they do not present antigens to T cells. Instead MICA and MICB can activate immune responses by engaging the NKG2D receptor on natural killer (NK) cells CD8+ T cells and gamma-delta T cells.
Biological function summary
MICA and MICB contribute to the immune surveillance system by marking stressed or transformed cells for destruction. These proteins are not part of a complex but are known ligands for the NKG2D receptor. Their interaction with NKG2D triggers the cytotoxic activity of NK cells and CD8+ T cells enhancing the destruction of cancerous or infected cells. Especially interesting is their role in tumor immunology where they serve as a danger signal to the immune system pointing NK cells toward tumor cells that would otherwise evade detection.
Pathways
The role of MICA and MICB centers around the immune response and cell stress pathways. One important pathway is the stress-induced signal transduction which upregulates these proteins in response to cellular stress such as heat shock. They fit into the immune surveillance pathway interacting with proteins like the NKG2D receptor and other signaling molecules that modulate immune cell activation. The pathway emphasizes the removal of damaged or abnormal cells and this interaction orchestrates timely immune responses.
MICA and MICB have associations with certain cancers like liver and colorectal cancers where they become over-expressed and shed from tumor cells to escape immune detection. Additionally their presence links to autoimmune diseases such as rheumatoid arthritis where the immune system attacks the body's own cells unjustly. The shedding of MICA/MICB from tumor cells involves enzymes like metalloproteinases affecting the engagement of NKG2D and complicating the immune response to cancerous cells.