Abcam, ab222407, Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160]

Size: 100µL / 1mL
Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407) is a rabbit monoclonal antibody detecting cleaved N-terminal DFNA5 / GSDME in Western Blot . Suitable for Mouse . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20866-160,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Mouse samples.
What is the molecular weight of cleaved N-terminal DFNA5 / GSDME?
Anti-cleaved N-terminal DFNA5 / GSDME [EPR20866-160] (ab222407) specifically detects a band for cleaved N-terminal DFNA5 / GSDME (UniProt: Q9Z2D3) at a molecular weight of approximately 37 kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl.
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Other related products
We have a range of other formats of antibody clone [EPR20866-160] also available for your convenience: ab222407, Carrier free -
ab256122
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The cleaved N-terminal DFNA5 also known as GSDME is a significant player in cellular processes. Upon cleaving it turns into an active fragment that carries out distinct functions. GSDME is an effector of necrotic cell death especially pyroptosis with a molecular mass of around 60 kDa. It resides within the cytoplasm of cells displaying expression in various tissues such as the colon stomach and esophagus. This protein functions as a security mechanism modulating cellular responses to stress and damage.
Biological function summary
The cleaved N-terminal fragment of GSDME disrupts the cell membrane using its pore-forming ability. GSDME belongs to the gasdermin family and becomes activated by caspase-3 a well-known executioner of apoptosis. It forms a part of a complex cellular machinery influencing the immune response and cell death. The action of GSDME in the apoptosis or pyroptosis decision is critical since it helps balance cell death pathways and inflammation preventing uncontrolled cell proliferation or excessive inflammation.
Pathways
The cleaved N-terminal GSDME integrates into the intrinsic apoptosis pathway acting downstream of caspase-3 activation. GSDME influences the balance between apoptosis and pyroptosis a critical decision point for cell fate in response to intracellular stress. It works in concert with other gasdermin family proteins such as GSDMD which plays a primary role in pyroptosis. The interaction between these proteins allows cells to select appropriate death pathways under different conditions revealing GSDME's versatility in cellular functioning.
The function of cleaved N-terminal GSDME links to hearing loss and various cancers. Mutations in GSDME associate with autosomal dominant forms of hearing loss highlighting its role in cochlear function. Furthermore low expression levels of GSDME have connections with cancer cell survival and proliferation. This protein interacts with proteins like caspase-3 in cancer where the cleavage of GSDME may promote pyroptosis over apoptotic pathways offering insights into potential therapeutic targets for tumor management.