Abcam, ab222485, Anti-Claudin 3 antibody [EPR19971] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Claudin 3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19971,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), IHC-P, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab222485 is the carrier-free version of
ab214487
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein 'Claudin 3' also known simply as Claudin-3 or CLDN3 is an important component of tight junctions in epithelial and endothelial cells. Claudin 3 has a molecular weight of approximately 22 kDa. It is expressed in a variety of tissues including the liver lungs and kidneys. By forming channels within tight junctions Claudin 3 regulates paracellular transport and maintains the barrier properties of cell layers thereby playing a significant role in preserving cellular environments.
Biological function summary
Claudin 3 contributes to the establishment and function of tight junctions that create a selective barrier in epithelial cell layers. This protein works as part of a larger complex consisting of other claudin family members which together ensure the integrity and permeability of the barrier. Through these complexes Claudin 3 helps facilitate cell polarity and tissue homeostasis which are essential for normal cellular function and organization.
Pathways
Claudin 3 participates in signaling pathways such as the Wnt/beta-catenin pathway and the epidermal growth factor receptor (EGFR) pathway. Claudin 3 modulates these pathways alongside other claudin proteins and partners like zonula occludens proteins. These interactions are central to processes like tissue morphogenesis and wound healing where precise control of cellular communication and migration is required.
Claudin 3 is linked to various cancers including ovarian and prostate cancer. Its expression is often altered in tumor cells where its dysregulation can lead to the disruption of tight junctions and contribute to cancer progression. In ovarian cancer Claudin 3 interacts with matrix metalloproteinases (MMPs) influencing the metastatic potential of cancer cells. Understanding Claudin 3's role can provide insights into new therapeutic targets for managing epithelial barrier dysfunction in cancer.