Abcam, ab222505, Mouse IL-9 ELISA Kit

Size: 1 x 96Tests
Mouse IL-9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-9 with a sensitivity of 1.67 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.67 pg/mL,
Range:3.1 - 200 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse IL-9 ELISA Kit (ab222505) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-9 protein in cit plasma, hep plasma, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-9 with 1.67 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-9 is a cytokine produced by CD4+ helper T cells, and it regulates hematopoietic cells through cell proliferation and the prevention of apoptosis.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Interleukin-9 (IL-9) sometimes known as a T-cell growth factor is a cytokine with a molecular mass of approximately 14-19 kDa. It is primarily expressed by T helper cells particularly Th2 and Th9 cells. IL-9 comes from the IL-9 gene and plays a significant role in the immune system by affecting various cell types such as mast cells B cells and epithelial cells. Researchers first identified it in the late 1980s and it has continued to be an area of interest due to its diverse functions.
Biological function summary
IL-9 influences immune responses and is important for various processes including cell proliferation and survival. IL-9 activates mast cells which play role in allergy response. It also affects the growth and differentiation of T cells and enhances the function of dendritic cells. IL-9 operates individually and not as part of a larger complex but works closely with its receptor IL-9R to initiate downstream signaling events.
Pathways
Cytokine signaling pathways and JAK-STAT signaling pathways integrate IL-9's function in the immune system. IL-9 activates the JAK-STAT pathway particularly involving proteins like JAK1 and STATs to relay signals that promote immune cell growth and survival. This cytokine also interlinks with other cytokines such as IL-4 and IL-13 playing role in immune regulation and inflammatory responses.
IL-9 shows strong correlation with asthma and certain types of cancers particularly Hodgkin's lymphoma. In asthma overproduction of IL-9 can lead to increased mucus production and airway hyperresponsiveness. This relationship makes it a potential target for blocking therapies in asthma treatment. Higher levels of IL-9 correlate with poorer prognosis in some cancers indicating its potential role in tumor growth and immune evasion possibly interacting with IL-6 in such conditions.