Abcam, ab222510, Human VEGF ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human VEGF ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human vascular endothelial growth factor (VEGF) with a sensitivity of 2.7 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader - Different formats for different needs including 384-well plate for higher throughput - Design your own immunoassay: we also offer the cojugation-ready antibody pair - Cited in over 30 publications
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Saliva, Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.7 pg/mL,
Range:12.5 - 800 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human VEGF ELISA Kit ab222510 is a rapid single-wash 90-min ELISA kit to measure Human VEGF in cerebral spinal fluid, saliva, urine, cell culture extracts, cell culture supernatant, milk, serum and plasma. This SimpleStep ELISA® sensitivity is 2.7 pg/mL.
How the assay works
Human VEGF SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Serial dilutions of recombinant human VEGFR1, VEGFR2, and VEGFR3 were prepared starting at 4 ng/mL and tested for interference. No interference was observed.
This VEGF immunoassay is calibrated against a highly purified human VEGF. The NIBSC/WHO unclassified purified human VEGF preparation 02/286 was evaluated in this kit. The dose response curve of the unclassified standard 02/286 parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep human VEGF kit to approximate NIBSC 02/286 units, use the equation below.
NIBSC 02/286 approximate value (IU/mL) = 0.0011 x SimpleStep human VEGF value (pg/mL).
Human VEGF ELISA Kit ab222510 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human VEGF ELISA kit ab222510
Human VEGF ELISA kit ab222510 has been used to assess endometrial development and receptivity
and to study VEGF as a predictor of arrhythmia in acute coronary syndrome patient
References:
Pin-Yao Lin et al. 2023, PMID:37763186,
Bin Li et al. 2023, PMID: 3735044
Save your precious samples
Our 384-well Human VEGF ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human IL-12 p70 Antibody Pair - BSA and Azide free
ab244013
- Anti-VEGFA antibody [EPR24630-77] - BSA and Azide free (Capture)
ab312861
- Anti-VEGFA antibody [EPR24630-90] - BSA and Azide free (Detector)
ab312862
Related and recommended products
Human VEGF ELISA kit ab222510 is commonly used to measure VEGF as a marker of angiogenesis.
Other ELISA kits to measure growth factors are:
- Human EGF ELISA kit
ab217772
- Human PEDF ELISA kit
ab246535
- Human HGF ELISA kit
ab275901
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C