Abcam, ab222838, Anti-STIL/SIL antibody

Size: 50µL
Rabbit Polyclonal STIL/SIL antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STIL aa 350-650.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human STIL aa 350-650. The exact immunogen used to generate this antibody is proprietary information.Q15468

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes->95%, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
STIL also known as SIL (SCL/TAL1 Interrupting Locus) is a protein involved mechanically in cellular processes that are important for cell division. STIL has a molecular mass of approximately 150 kDa. This protein localizes primarily in the nucleus where it is abundantly expressed in actively dividing cells. It plays a direct role in maintaining proper cell cycle progression especially during mitosis.
Biological function summary
The STIL protein plays a role in regulating centrosome duplication ensuring accurate chromosome segregation. STIL forms part of a complex with other proteins like SAS-6 which is important in forming the mitotic spindle apparatus. This action is essential to maintaining the structural integrity of centrosomes which are key for correct cell division.
Pathways
The STIL protein integrates into cell cycle regulation and the centrosome cycle. STIL interacts with proteins such as PLK4 and CEP135 within the centrosome duplication pathway. This interaction is important for the initiation of procentriole biogenesis aligning with the G1 phase of the cell cycle.
Misregulation of the STIL protein associates strongly with cancer and microcephaly. For instance overexpression of STIL links to various cancers as it can lead to abnormal cell proliferation. Additionally mutations in the STIL gene are connected to microcephaly a condition characterized by underdeveloped cerebral cortex. Here STIL mutation affects proteins like MCPH1 which is involved in brain development.