Abcam, ab223268, Anti-ENPP1/PC1 antibody [EPR22262-22]

Size: 100µL / 1mL
Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268) is a rabbit monoclonal antibody detecting ENPP1/PC1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22262-22,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, IP, ICC/IF, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of ENPP1/PC1?
Anti-ENPP1/PC1 [EPR22262-22] (ab223268) specifically detects a band for ENPP1/PC1 (UniProt: P22413) at a molecular weight of 105kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR22262-22] also available for your convenience: ab223268, Carrier free -
ab240832
, Alexa Fluor® 488 -
ab312900
, Alexa Fluor® 647 -
ab315846
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ENPP1 also known as PC-1 is a type II transmembrane glycoprotein with a molecular weight of approximately 130 kDa. It is expressed in various tissues including the liver kidney and bone. ENPP1 exhibits the enzymatic activity of nucleotide pyrophosphatase/phosphodiesterase which hydrolyzes ATP to AMP and diphosphate. This activity is important for the regulation of extracellular nucleotide levels and mineralization processes. Researchers often utilize the ENPP1 assay to study its function and activity.
Biological function summary
ENPP1 significantly influences bone mineralization and soft tissue calcification. It interacts with proteins like ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1) impacting cellular uptake of calcified materials. The protein is also involved in the regulatory networks responsible for controlling insulin signaling impacting metabolic processes. ENPP1 may form complexes with other proteins which can finely tune its biological activity and effects.
Pathways
ENPP1 plays a role in the insulin signaling pathway and the pyrophosphate pathway. Via its participation in insulin signaling ENPP1 regulates glucose metabolism and influences insulin sensitivity. It affects pathways related to mineral metabolism where it interacts with proteins like ENPP3. By regulating extracellular pyrophosphate levels ENPP1 contributes to inorganic phosphate homeostasis and influences skeletal development and maintenance.
ENPP1 has associations with type 2 diabetes and generalized arterial calcification of infancy (GACI). Its role in type 2 diabetes relates to its ability to inhibit insulin receptor function therefore contributing to insulin resistance. ENPP1 also connects to proteins involved in vascular calcification such as matrix Gla protein (MGP) in the context of GACI. These associations highlight the importance of ENPP1 in metabolic and calcification disorders.