Abcam, ab223587, Mouse/Rat FGF1 ELISA Kit

Size: 1 x 96Tests
Mouse/Rat FGF1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat FGF1 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Urine, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse, Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 2 pg/mL,
Range:16 - 1000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse/Rat FGF1 ELISA Kit (ab223587) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF1 protein in cit plasma, edta plasma, serum, tissue lysate, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat FGF1 with NULL NULL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
FGF1, also known as Fibroblast Growth Factor 1 or FGF acidic, is an important activator for several signaling cascades with roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. It functions as potent mitogen in vitro. In the presence of heparin, FGF1 binds in a 1:2 stoichiometry with FGF1 receptor protein, causing the autophosphorylation and activation of the receptor. This complex then binds to integrin and recruits PTPN11 to begin FGF1 signaling. Because of its possible role in organ development and in tumor growth, FGF1 is an important subject of study for the treatment of diabetes and the diagnosis and treatment of cancer. Mouse and rat FGF1 are identical on an amino acid level.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FGF1 also known as acidic fibroblast growth factor (aFGF) is a member of the fibroblast growth factor family. This protein has a molecular mass of approximately 17 kDa. FGF1 is expressed in a variety of tissues such as the brain liver and heart and plays an essential role in cell growth development and repair. The FGF1 protein is sometimes studied using techniques such as FGF1 ELISA which helps in quantifying and understanding its expression levels and activity in different contexts.
Biological function summary
FGF1 is involved in numerous cellular processes beyond simple cell proliferation. It binds to fibroblast growth factor receptors (FGFRs) on the cell surface initiating a cascade of downstream signaling events. This protein does not act alone; it is often a part of larger protein complexes that modulate cellular processes such as angiogenesis and wound healing. FGF1 is also implicated in nerve cell differentiation and survival through FGFR-mediated pathways.
Pathways
The FGF1 protein is integrally involved in the MAPK/ERK signaling pathway a critical pathway that regulates cell division growth and differentiation. Through this pathway FGF1 interacts with various proteins including Ras Raf and ERK to propagate the signal necessary for mitogenic and survival responses. FGF1 is also a player in the phosphoinositide 3-kinase (PI3K) pathway which helps control cellular responses to stress and metabolic regulation showing its role in cellular homeostasis.
FGF1 is associated with conditions such as cancer and cardiovascular diseases. The deregulation of FGF1 expression or its signaling pathways can lead to tumor development and progression with FGF1 often interacting with other proteins like the basic fibroblast growth factor (bFGF) in these contexts. In cardiovascular diseases altered FGF1 signaling might contribute to atherosclerosis and cardiac repair processes where its interaction with other growth factors like vascular endothelial growth factor (VEGF) is significant.