Abcam, ab223592, Human IL-12 p70 ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human IL-12 p70 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-12 p70 with a sensitivity of 13.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 13.6 pg/mL,
Range:31.3 - 2000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IL-12 p70 ELISA Kit ab223592 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-12 p70 in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 13.6 pg/mL.
How the assay works
Human IL-12 p70 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human IL-12 p70 ELISA Kit ab223592 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
IL-12 p70 is a 70kDa cytokine heterodimer made of 2 subunits IL-12A (p35) and IL-12B (p40). This kit recognizes specifically the IL-12 total heterodimer protein. IL-12 p70 is produced by active antigen-presenting cells and promotes the development of Th1 and induces IFN?.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C