Abcam, ab223864, Human Von Willebrand Factor ELISA Kit

Size: 1 x 96Tests / 10 x 96Tests
Human Von Willebrand Factor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Von Willebrand Factor with a sensitivity of 0.079 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 10 citations
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human, Rhesus monkey,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.079 ng/mL,
Range:0.469 - 30 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Human Von Willebrand Factor ELISA Kit ab223864 is a rapid single-wash 90-min Sandwich ELISA to measure Human Von Willebrand Factor in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.079 ng/mL.
How the assay works
Human Von Willebrand Factor SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Von Willebrand Factor ELISA Kit ab223864 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Von Willebrand Factor antibody [EPR20144-204] - BSA and Azide free (Capture)
ab244932
- Anti-Von Willebrand Factor antibody [EPR20144-257] - BSA and Azide free (Detector)
ab244932
Von Willebrand Factor is critical in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Von Willebrand Factor also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C