Abcam, ab224094, Anti-DDX47 antibody - C-terminal

Size: 100µL
Rabbit Polyclonal DDX47 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDX47 aa 300 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human DDX47 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9H0S4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDX47 also known as DEAD box protein 47 functions as a putative RNA helicase involved in the unwinding of RNA secondary structures. This protein weighing approximately 52 kDa contains the conserved D-E-A-D amino acid motif characteristic of DEAD box proteins. It expresses broadly across various tissues but shows significant levels in the nucleolus where it plays roles in nucleolar activities.
Biological function summary
This protein participates in ribosome biogenesis by assisting in the maturation of ribosomal RNA. DDX47 as part of ribonucleoprotein complexes facilitates the cleavage of precursor rRNA which is necessary for the production of functional ribosomal subunits. This process is essential for maintaining high rates of protein synthesis in rapidly growing cells thereby influencing cellular growth and proliferation.
Pathways
RNA metabolism and ribosome biogenesis include DDX47 as an important component. In these pathways it interacts with proteins such as NOP56 and NOLC1 which are integral to ribosomal RNA processing. Through its role in these pathways DDX47 influences transcriptional and translational regulation which are fundamental processes in cell physiology.
Alterations in DDX47 expression link to cancer and ribosomopathies. Abnormal activity or dysregulation of DDX47 can affect cell proliferation contributing to tumorigenesis. This protein connects to oncogenic proteins such as MYC which is associated with cell cycle regulation and cancer progression. Understanding these interactions provides insights into how DDX47 may contribute to disease pathogenesis and presents potential diagnostic or therapeutic targets.