Abcam, ab224536, Anti-TRNT1 antibody

Size: 100µL
Rabbit Polyclonal TRNT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRNT1 aa 300-400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human TRNT1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.Q96Q11

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRNT1 also known as tRNA nucleotidyl transferase 1 is a protein that attaches the CCA sequence to the 3' end of tRNA molecules. This addition is essential for transferring amino acids during protein synthesis. TRNT1 has a molecular mass of about 48 kDa. The protein is expressed in many tissues but is particularly abundant in metabolically active tissues like heart and liver where protein synthesis is high.
Biological function summary
The addition of the CCA tail is a necessary step at the end of tRNA maturation. Without this tRNA molecules cannot function in protein synthesis. TRNT1 does not work alone; it complements the function of other tRNA modifying enzymes adding the correct nucleotides to tRNA. TRNT1 frequently interacts with other molecules to ensure the processing is accurate and efficient.
Pathways
TRNA CCA addition by TRNT1 plays a role in RNA processing and protein synthesis pathways. These pathways are fundamental for cell function impacting how cells make proteins. TRNT1 interacts with other RNA-related proteins like tRNA synthetases which help link tRNA to specific amino acids. This interaction shows the connectedness of TRNT1 with proteins involved in translational machinery.
Improper function of TRNT1 associates with sideroblastic anemia with B-cell immunodeficiency periodic fevers and developmental delay (SIFD). In this context inadequate TRNT1 activity leads to defective tRNA maturation contributing to disease symptoms. TRNT1’s malfunction can also relate to other proteins in these diseases such as mutant forms of certain mitochondrial proteins exacerbating clinical features.