Abcam, ab224702, Anti-MICA + MICB antibody [EPR22031]

Size: 100µL / 1mL
Anti-MICA + MICB antibody [EPR22031] (ab224702) is a rabbit monoclonal antibody detecting MICA + MICB in Western Blot, IHC-P . Suitable for Human, . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22031,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-MICA + MICB antibody [EPR22031] (ab224702) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, samples.
What is the molecular weight of MICA + MICB?
Anti-MICA + MICB [EPR22031] (ab224702) specifically detects a band for MICA + MICB (UniProt: Q29983) at a molecular weight of 42kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-MICA + MICB antibody [EPR22031] (ab224702) has been confirmed by Western blot testing in MICA Knockout A549 cells.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MICA and MICB also known as MIC-A and MIC-B are stress-induced proteins with significant roles in immune responses. These proteins are widely known for their presence on the surface of many human cell types most notably epithelial and endothelial cells. MICA is a glycoprotein with a molecular mass of approximately 70 kDa while MICB has a similar structure and mass. They are both part of the MHC class I-like molecules and function as ligands to the NKG2D receptor on natural killer (NK) cells and certain T cells a critical interaction for immune surveillance.
Biological function summary
MICA and MICB act in the activation of the immune system by modulating NK cells and CD8+ T cell activities. These proteins do not form part of a complex but operate independently to signal the presence of infected or transformed cells to the immune system. They often appear on the cell surface under stress such as during viral infections or cancerous transformations activating immune cells to target and destroy these abnormal cells.
Pathways
The MICA/MICB interaction with NKG2D is integral to the immune checkpoint pathway. This pathway plays a significant role in regulating the immune system's response to aberrant cells. Additionally MICA and MICB activity is linked to the regulation of the cytokine IFN-γ promoting the recruitment and activation of immune effector cells. The roles of these proteins in these pathways underline their importance in maintaining immune homeostasis and responding to pathological challenges.
MICA and MICB have notable associations with cancer and autoimmune diseases. Aberrant expression of these proteins on tumor cells facilitates their recognition and elimination by immune cells linking MICA and MICB directly to tumor immunosurveillance. In autoimmune disorders improper regulation of MICA/MICB expression can lead to undesirable immune cell activation contributing to conditions like rheumatoid arthritis. The proteins connect with others like NKG2D in both cancer immunity and autoimmune diseases highlighting their dual role in health and disease.