Abcam, ab224881, Human TREM2 ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human TREM2 ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human TREM2 with a sensitivity of 10.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Need to design your own assay: we also offer the conjugation-ready antibody pair - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 10.5 pg/mL,
Range:78.1 - 5000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human TREM2 ELISA Kit ab224881 is a rapid single-wash 90-min ELISA kit to measure Human TREM2 in cerebral spinal fluid (CSF), cell culture extracts, tissue extracts, cell culture media, serum and plasma. This SimpleStep ELISA® sensitivity is 10.5 pg/mL.
How the assay works
Human TREM2 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant Human TREM2 protein in serum, plasma, cerebrospinal fluid and cell culture supernatant, cell and tissue extract samples only.
Human TREM2 ELISA Kit ab224881 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human TREM2 ELISA kit ab224881
Human TREM2 ELISA kit ab224881has been used to study the blood brain barrier disruption in anti-NMDA receptor encephalitis
and to study TREM2 expression in plasma and CSF of Alzheimer disease patients
References:
Haoxiao Chang et al. 2023, PMID: 37481571,
F. La Rosa et al. 2023, PMID: 37239970
Save your precious samples
Our 384-well Human TREM2 ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human TREM2 Antibody Pair - BSA and Azide free
ab244027
- Anti-TREM2 antibody [EPR20243-78] - BSA and Azide free (Capture)
ab244673
- Anti-TREM2 antibody [EPR20243-269] - BSA and Azide free (Detector)
ab244938
Related and recommended products
Human TREM2 ELISA kit ab224881 is commonly used to measure TREM2 as a marker of neurodegenerative disorders.
Other ELISA kits to study neurodegeneration include:
- Human IL-6 ELISA kit
ab17801
- Human CSF-1-R ELISA kit
ab230940
TREM2 is immune receptor expressed on the surface of microglia. TREM2 is proteolytically process by ADAM proteases and the soluble TREM2 is released into the extra cellular space. Elevated levels of soluble TREM2 in cerebrospinal fluid is a biomarker associated with increased risk of Alzheimer's disease. ab224881 TREM2 SimpleStep ELISA
is designed against the extracellular portion of TREM2.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C