Abcam, ab225196, Alexa Fluor® 647 Anti-Zic2 antibody [EPR7790]

Size: 100µL
Rabbit Recombinant Monoclonal ZIC2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7790,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Zic2 also called Zinc finger protein of cerebellum 2 represents a transcription factor notable for its role in the regulation of gene expression. Mechanically Zic2 binds to DNA sequences via its zinc finger domains facilitating transcriptional activation or repression depending on the context. This protein has an approximate molecular mass of 60 kDa and exhibits expression mainly in the developing central nervous system. Zic2 shows high levels of expression in postmitotic neurons and various embryonic tissues indicating its involvement in central neural developmental processes.
Biological function summary
Zic2 impacts the differentiation and proliferation of neural progenitor cells. Zic2 functions as part of a transcriptional regulatory complex collaborating with other partners to control genes necessary for neurodevelopment. Its activity supports the specification of neuronal identity and axonal pathfinding. Studies demonstrate its important role in guiding axons to their correct targets a function integral during the formation of neural circuits. Zic2-mediated transcriptional networks are essential for accurate neuronal patterning in the central nervous system.
Pathways
Several interactions revolving around Zic2 play essential roles. Zic2 is involved in the Sonic Hedgehog (Shh) signaling pathway where it contributes to the transcriptional response important for patterning activities. Within the Shh pathway Zic2 interacts with downstream components like Gli proteins which modulate target gene expression based on signal gradient. Additionally Zic2 participates in the Wnt signaling pathway where it influences cell fate determination and tissue polarity. Such pathways highlight its part in developmental processes and cellular differentiation.
Mutations or dysregulation of Zic2 often associate with congenital malformations such as holoprosencephaly and mirror movement disorder. These disorders arise from disrupted neural development reflecting Zic2's key roles in morphogenesis and patterning. Both conditions involve abnormal neurodevelopment where altered Zic2 interactions with proteins such as Gli3 result in impaired signaling cascades. Understanding these connections helps clarify Zic2's contribution to pathological states offering insights into potential therapeutic targets.