Abcam, ab225311, Alexa Fluor® 647 Anti-RNA Helicase A antibody [EPR13521]

Size: 100µL
Rabbit Recombinant Monoclonal RNA Helicase A antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13521,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNA Helicase A also known as DHX9 is a protein essential for various cellular processes. This helicase protein belongs to the DEXH-box family and facilitates the unwinding of RNA structures which is critical for RNA metabolism. The molecular weight of RNA Helicase A is approximately 141 kDa. It is expressed in a variety of human tissues including the nucleus cytoplasm and sometimes nucleoli. The protein’s helicase activity is central to its mechanical role in cellular functioning.
Biological function summary
RNA Helicase A is involved in numerous processes such as transcription RNA processing and the assembly of ribonucleoprotein complexes. The protein plays an important role in facilitating RNA polymerase II transcription and also has functions in RNA splicing through its association with spliceosomal components. RNA Helicase A does not function in isolation; instead it forms part of multi-protein complexes that are necessary for its diverse cellular roles. It interacts with various proteins to achieve its functions impacting both RNA and DNA substrates.
Pathways
RNA Helicase A influences the regulation of gene expression and is a significant player in the innate immune response. It participates in the Toll-like receptor (TLR) signaling pathway where it helps detect viral RNA and triggers immune responses. It also interacts with proteins like TLR3 and TRIF which are involved in the signaling cascade essential for producing type I interferons and pro-inflammatory cytokines.
RNA Helicase A has associations with cancer and viral infections. Abnormal regulation of RNA Helicase A has been linked with tumorigenesis where its altered expression contributes to oncogenic processes. It also plays a part in the host response to viral infections where the protein aids in recognizing viral nucleic acids. RNA Helicase A interacts with viral proteins thereby influencing viral replication and host antiviral responses. These connections highlight the protein’s relevance in both cancer biology and infectious disease pathogenesis.