Abcam, ab225440, Alexa Fluor® 647 Anti-active YAP1 antibody [EPR19812]

Size: 100µL
Rabbit Recombinant Monoclonal YAP1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Mouse samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19812,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Mouse,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab225440 is specific to the active (non-phosphorylated) form of YAP1.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle|Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Active YAP1 also known as Yes-associated protein 1 is a transcription co-activator with a mass of approximately 65 kDa. It plays a mechanical role in the regulation of gene expression by interacting with transcription factors and enhancing their ability to drive transcription. YAP1 is expressed in various tissues including the liver intestine and neural tissues. Also found in the cytoplasm and nucleus its expression is tightly regulated because of its involvement in maintaining cell proliferation and survival.
Biological function summary
Active YAP1 functions as an important regulator in the Hippo signaling pathway. It acts as an effector that transmits signals from upstream kinases to control organ size by modulating cell growth and apoptosis. YAP1 associates with the TEAD transcription factor family to regulate the expression of genes involved in cell proliferation and differentiation. The complex formed between YAP1 and TEAD is essential for its biological activity influencing processes like stem cell renewal and tissue regeneration.
Pathways
Active YAP1 has a central role in the Hippo signaling pathway important for controlling cellular behaviors such as growth and survival. In this pathway YAP1 interacts with other proteins like LATS1/2 kinases to control its activity through phosphorylation and subcellular localization. Akt/mTOR pathway also connects with YAP1 integrating signals that affect cell growth and metabolism. These interactions highlight YAP1’s position as a pivotal node in maintaining cellular homeostasis.
Active YAP1 is implicated in several conditions including cancer and liver disorders. Overexpression or dysregulation of YAP1 leads to oncogenic transformation closely associating it with cancers such as hepatocellular carcinoma and breast cancer. In liver disorders YAP1's activity affects fibrosis and regeneration by interacting with proteins like β-catenin modulating pathways linked to tissue repair and scarring. Understanding YAP1’s role offers potential therapeutic targets for managing these diseases.