Abcam, ab225905, Anti-ANKLE2 antibody

Size: 100µL
Rabbit Polyclonal ANKLE2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ANKLE2 aa 800-850.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ANKLE2 aa 800-850. The exact immunogen used to generate this antibody is proprietary information.Q86XL3

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab225905 was affinity purified using an epitope specific to ANKLE2 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ANKLE2 also known as Ankyrin Repeat And LEM Domain Containing 2 is a protein with an approximate mass of 112 kDa. Mechanically ANKLE2 plays a role in regulating nuclear envelope reconstruction during cell division. It is expressed in various tissues including the brain liver and heart. ANKLE2 locates in the nucleus and cytoplasm highlighting its engagement in cellular functions related to the nuclear envelope and mitosis.
Biological function summary
ANKLE2 contributes to maintaining nuclear integrity and proper cell cycle progression. It does not form part of a known complex but interacts closely with other nuclear envelope proteins. ANKLE2 helps in the disassembly and reassembly of the nuclear envelope supporting chromosome segregation and cell division. Its activity ensures the correct formation of nuclear structures post-mitosis preventing potential errors that could disrupt cellular functions.
Pathways
ANKLE2 is involved in the mitotic spindle checkpoint pathway and cell cycle control pathway. It interacts with proteins like BAF (Barrier to Autointegration Factor) in these pathways which are essential for nuclear envelope reformation. ANKLE2’s functioning in these pathways demonstrates its participation in monitoring and ensuring successful cell cycle transitions particularly during the mitotic phase.
ANKLE2 is associated with microcephaly and other neurodevelopmental disorders. Mutations in ANKLE2 have connections to impaired brain development highlighting its significance in neural progenitor cell division. ANKLE2 interacts with Zika virus proteins which has been linked to microcephaly resulting from viral infection. Further research into ANKLE2 and related proteins could provide insights into preventing and treating these conditions.