Abcam, ab226259, Anti-AlaRS antibody

Size: 100µL
Rabbit Polyclonal AlaRS antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AARS1 aa 250-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AARS1 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.P49588

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab226259 was affinity purified using an epitope specific to AlaRS immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AlaRS also known as alanyl-tRNA synthetase is an enzyme critically involved in protein synthesis. It has an approximate molecular mass of 100 kDa. AlaRS catalyzes the attachment of alanine to its corresponding tRNA molecule ensuring the incorporation of alanine into a growing polypeptide chain. This enzymatic activity makes it an important component in the translation machinery. AlaRS shows expression across a wide variety of tissues reflecting its essential role in cellular function and biosynthesis.
Biological function summary
The enzyme plays an essential role without functioning as part of a complex. AlaRS participates in the aminoacylation of tRNA a process ensuring fidelity during protein translation. It charges tRNA with alanine maintaining accuracy in protein synthesis. This process is vital for cellular function because incorrect amino acid incorporation can lead to misfolded proteins and malfunction.
Pathways
AlaRS plays an important part in the translation pathway an essential component of the wider protein biosynthesis network. It interacts closely with ribosomal proteins during translation. AlaRS also links to the aminoacyl-tRNA biosynthesis pathway sharing functional similarities with other aminoacyl-tRNA synthetases such as GlnRS and GlyRS that attach amino acids to their corresponding tRNAs.
AlaRS has connections with Charcot-Marie-Tooth disease and specific types of cancer. Mutations in the gene encoding AlaRS can lead to Charcot-Marie-Tooth disease a disorder affecting peripheral nerves. The enzyme also shows altered expression in some cancers connecting it indirectly with oncogenic proteins that contribute to tumor progression. These associations highlight AlaRS as a target for understanding pathologies related to protein synthesis dysregulation.