Abcam, ab226483, Anti-GSDMB antibody [EPR20841] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GSDMB antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20841,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:According to collaborator's data, this antibody recognizes both full-length and N-term GSDMB (PMID: 32299851).

Product details:
ab226483 is the carrier-free version of
ab215729
This antibody was developed in collaboration with Dr Feng Shao's lab, NIBS.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GSDMB also known as Gasdermin-B is a protein that plays a significant role in cellular mechanisms. With a molecular weight of approximately 52 kDa GSDMB is encoded by the GSDMB gene. This protein belongs to the gasdermin family and is expressed in various tissues including the stomach esophagus and gastrointestinal tract. Its expression pattern suggests it might have specialized roles in these particular tissues.
Biological function summary
Gasdermin-B is involved in cell death processes particularly in pyroptosis which is a form of programmed cell death. GSDMB forms oligomeric complexes that create pores in the lipid bilayer of cellular membranes. These pores lead to cell swelling and lysis contributing to the inflammatory response. GSDMB also interacts with other gasdermin family proteins indicating a collaborative role in controlling cell death and inflammation.
Pathways
GSDMB is an important component of the pyroptosis pathway and the regulation of immune responses. It interacts with specific members of the caspase family especially Caspase-1 which activates Gasdermin-D in a similar pathway. GSDMB modulates inflammatory signaling by assisting in the release of pro-inflammatory cytokines through its pore-forming activity linking GSDMB to processes that maintain homeostasis and immune defense.
Gasdermin-B has been linked to various inflammatory and autoimmune conditions such as Crohn's disease and ulcerative colitis. Altered expression levels of GSDMB can influence disease severity and progression. Additionally GSDMB interacts with TNF-alpha a cytokine involved in the immune response highlighting its importance in inflammatory disease environments. Exploring GSDMB's role in these conditions can advance therapeutic strategies for treating such disorders.