Abcam, ab227535, Anti-CREST antibody

Size: 100µL
Rabbit Polyclonal CREST antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SS18L1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IP, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SS18L1. The exact immunogen used to generate this antibody is proprietary information.O75177

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CREST also known as SS18L1 is a 406 amino acid protein with a molecular weight of approximately 46 kDa. It is widely expressed in the central nervous system particularly in neurons. As a member of the SWI/SNF chromatin remodeling complex CREST plays a significant role in transcriptional regulation by altering chromatin architecture. Researchers often utilize CREST antibodies in experiments to study its localization and expression levels with CREST staining being a useful technique in identifying its presence in neuronal tissues.
Biological function summary
CREST functions as a regulator of gene transcription which is essential for various neuronal activities such as dendritic development and synaptic plasticity. As a component of the SWI/SNF complex CREST interacts with other proteins to modulate the transcription of genes required for neuronal differentiation and activity-dependent responses. These interactions help to facilitate the structural and functional modifications necessary for synaptic development and neurotransmission.
Pathways
CREST interacts within the calcium signaling pathway and CREB signaling pathways. In the calcium signaling pathway CREST has a role in calcium-dependent regulation of gene expression impacting neuronal development. It operates closely with other proteins like CBP (CREB-binding protein) to regulate the transcription of genes that are important for neuronal plasticity and memory formation.
CREST is linked to Huntington's disease and autism spectrum disorders. In Huntington's disease CREST's role in gene transcription and synaptic plasticity may influence the progression of the disease particularly through interactions with huntingtin the protein mutated in this disorder. Similarly in autism spectrum disorders alterations in CREST function or expression may affect normal neuronal development and connectivity potentially involving connections with proteins such as MeCP2 which is implicated in neurodevelopmental disorders. CREST products including CREST antibodies serve as invaluable resources for investigating these connections in various biological and clinical studies.